Question 1

KM is

Accepted Answer

A)  a measure of the catalytic efficiency of the enzyme.
B)  equal to half of Vmax
C)  the rate constant for the reaction ES  →\rightarrow→  E + P.
D)  the [S] that half-saturates the enzyme.
E)  a ratio of substrate concentration relative to catalytic power. F) A) and B)
G) C) and D)

Question 2

The breakdown of dopamine is catalyzed by the enzyme monoamine oxidase (MAO) .What is the final concentration of product if the starting dopamine concentration is 0.050 M and the reaction runs for 5 seconds.(Assume the rate constant for the reaction is 0.249 s−1.)

Accepted Answer

A)  0.050 M
B)  0.014 M
C)  0.018 M
D)  1.2 M
E)  0.025 MF) C) and D)
G) A) and D)

Question 3

Matching
-A lead compound for a new drug should bind to its target protein with a very ______.

Accepted Answer

A)  isozymes
B)  [A]
C)  the rate constant
D)  Ping Pong
E)  bimolecularF) ES complexG) random orderedH) competitive inhibitionI) unimolecularJ) [A]²K) Competitive inhibitionL) phosphorylationM) small KSN) large KSO) uncompetitive inhibitionP) [B]Q) M) and P)
R) D) and N)

Question 4

Pseudo-first-order reaction kinetics would be observed for the reaction A + B  →\rarr→  C

Accepted Answer

A)  if [A] or [B] > [C].
B)  if [C]>[A] and [C]>[B].
C)  if [A] or [B] = 0.
D)  if [C] = 0.
E)  none of the above F) B) and E)
G) C) and E)

Question 5

A Lineweaver-Burk plot is also referred to as
I.a sigmoidal plot.
II.a linear plot.
III.a Michaelis-Menten plot.
IV.a double reciprocal plot.

Accepted Answer

A)  II
B)  II, III
C)  IV
D)  II, IV
E)  III, IVF) B) and E)
G) A) and B)

Question 6

Learning objective: Reaction Kinetics
The following questions refer to the diagram (with boxes where it has been left incomplete) : 
-Which of the following is correct in regards to the diagram above?

Accepted Answer

A)  X=A, Y=B, Z=P
B)  X=B, Y=A, Z=Q
C)  X=E, Y=A, Z=E
D)  X=E, Y=B, Z=Q
E)  X=E, Y=B, Z=PF) A) and B)
G) A) and D)

Question 7

________ clinical trials are focused on evaluating the efficacy of new drug candidates, and usually use _____ test.

Accepted Answer

A)  Phase 1; single blind
B)  Phase 1; double blind
C)  Phase 2; single blind
D)  Phase 2; double blind
E)  Phase 3; double blindF) A) and C)
G) C) and E)

Question 8

Matching
-Different enzymes that catalyze the same reaction, although may be found in different tissues, are known as ______.

Accepted Answer

A)  isozymes
B)  [A]
C)  the rate constant
D)  Ping Pong
E)  bimolecularF) ES complexG) random orderedH) competitive inhibitionI) unimolecularJ) [A]²K) Competitive inhibitionL) phosphorylationM) small KSN) large KSO) uncompetitive inhibitionP) [B]Q) C) and F)
R) M) and P)

Question 9

Enzyme E is responsible for conversion of substrate X to product U.As a result of this conversion electrons are transported to a coenzyme (FAD) within Enzyme E.In order for the reaction to be completed, a second substrate NAD+ must also bind Enzyme E and collect stored electrons (which converts it to product, NADH) .The graph below shows the data while varying X, with fixed concentrations of NAD+.What type of multi-substrate mechanism does enzyme E utilize? 
+ must also bind Enzyme E and collect stored electrons (which converts it to product, NADH) .The graph below shows the data while varying X, with fixed concentrations of NAD+.What type of multi-substrate mechanism does enzyme E utilize?    A) sequential - Ordered B) sequential - Random C) simultaneous addition D) Ping Pong E) Sequential but the data cannot differentiate between ordered and random." class="answers-bank-image d-inline" rel="preload" >

Accepted Answer

A)  sequential - Ordered
B)  sequential - Random
C)  simultaneous addition
D)  Ping Pong
E)  Sequential but the data cannot differentiate between ordered and random.F) A) and C)
G) B) and C)

Question 10

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the Vmax? max?   A) 0.24 μM/s B) 18 μM C) 0.2 μM D) 0.24 μM E) 0.12 μM/s" class="answers-bank-image d-inline" rel="preload" >

Accepted Answer

A)  0.24 μM/s
B)  18 μM
C)  0.2 μM
D)  0.24 μM
E)  0.12 μM/sF) C) and D)
G) B) and C)

Question 11

Learning objective: Reaction Kinetics
The following questions refer to the diagram (with boxes where it has been left incomplete) : 
-This diagram refers to a (an)

Accepted Answer

A)  Ping Pong reaction.
B)  ordered bisubstrate reaction.
C)  random bisubstrate reaction.
D)  double order ping pong reaction
E)  X, Y, and Z must be provided in order to answer correctlyF) C) and E)
G) None of the above

Question 12

The following questions refer to the overall transformation shown in the following reaction: 2>k1 and k−12. B) The reaction is zero order with respect to [S] if [S]>>[E] C) It describes a double displacement reaction D) All of the above are true. E) None of the above is true." class="answers-bank-image d-inline" rel="preload" >
-Which of the following is (are) true?

Accepted Answer

A)  The [ES] will remain constant if k2>k1 and k−12.
B)  The reaction is zero order with respect to [S] if [S]>>[E]
C)  It describes a double displacement reaction
D)  All of the above are true.
E)  None of the above is true.F) D) and E)
G) C) and E)

Question 13

Determine the KM and Vmax from the following graph.(Note: On the x-axis the minor tick mark spacing is 0.005; on the y-axis the minor tick mark spacing is 0.002)  M and Vmax from the following graph.(Note: On the x-axis the minor tick mark spacing is 0.005; on the y-axis the minor tick mark spacing is 0.002)    A) KM = [0.006]; Vmax = 0.0075/s B) KM = [0.196]; Vmax = 0.0075/s C) KM = [165]; Vmax = 33/s D) KM = [33]; Vmax = 167/s E) KM = [270]; Vmax x = 68/s" class="answers-bank-image d-inline" rel="preload" >

Accepted Answer

A)  KM = [0.006]; Vmax = 0.0075/s
B)  KM = [0.196]; Vmax = 0.0075/s
C)  KM = [165]; Vmax = 33/s
D)  KM = [33]; Vmax = 167/s
E)  KM = [270]; Vmax x = 68/sF) A) and B)
G) All of the above

Question 14

The following questions refer to the overall transformation shown in the following reaction: 1=k−1 B) implies that k−1 and k2 are such that the [ES] = k1[ES] C) [P]>>[E] D) [S] = [P] E) ES breakdown occurs at the same rate as ES formation" class="answers-bank-image d-inline" rel="preload" >
-For the reaction, the steady state assumption

Accepted Answer

A)  implies that k1=k−1
B)  implies that k−1 and k2 are such that the [ES] = k1[ES]
C)  [P]>>[E]
D)  [S] = [P]
E)  ES breakdown occurs at the same rate as ES formationF) C) and D)
G) All of the above

Question 15

An enzyme is near maximum efficiency when

Accepted Answer

A)  its turnover number is near Vmax.
B)  kcat/KM is near 108 M-1s-1.
C)  k1 -1.
D)  kcat/KM is equal to kcat.
E)  KM is large when k2 exceeds k1.F) A) and B)
G) A) and C)

Question 16

Enzyme activity in cells is controlled by which of the following?
I.covalent modifications
II.modulation of expression levels
III.feedback inhibition
IV.allosteric effectors

Accepted Answer

A)  I
B)  II
C)  III
D)  III, IV
E)  I, II, III, IVF) A) and C)
G) B) and D)

Question 17

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the KM? M?   A) 0.24 μM/s B) 18 μM C) 0.2 μM D) 0.24 μM E) 0.12 μM/s" class="answers-bank-image d-inline" rel="preload" >

Accepted Answer

A)  0.24 μM/s
B)  18 μM
C)  0.2 μM
D)  0.24 μM
E)  0.12 μM/sF) None of the above
G) A) and B)

Question 18

I propose to design a new drug which will act as an inhibitor for an enzyme.If I have used all current information about the mechanism of this enzyme to design this inhibitor and I carefully engineer it with similar chemical properties of the transition state, what type of inhibitor am I attempting to engineer and how will I know if I have succeeded?

Accepted Answer

A)  A competitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in Vmax.
B)  A competitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in KM.
C)  A uncompetitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in KM.
D)  A uncompetitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in Vmax.
E)  None of the above.F) A) and B)
G) A) and E)

Question 19

When [S] = KM,  υ\upsilonυ 0 = (_____) × (Vmax) .

Accepted Answer

A)  [S]
B)  0.75
C)  0.5
D)  KM
E)  kcat F) A) and D)
G) C) and D)

Question 20

Matching
-Assume a first order reaction, the rate of the reaction 2A →\rarr→ B is dependent on ______.

Accepted Answer

A)  isozymes
B)  [A]
C)  the rate constant
D)  Ping Pong
E)  bimolecularF) ES complexG) random orderedH) competitive inhibitionI) unimolecularJ) [A]²K) Competitive inhibitionL) phosphorylationM) small KSN) large KSO) uncompetitive inhibitionP) [B] Q) E) and J)
R) All of the above

Exam 12: Enzyme Kinetics, Inhibition and Control

Learning objective: Reaction Kinetics The following questions refer to the diagram (with boxes where it has been left incomplete) : -This diagram refers to a (an)

Correct Answer
verified

Matching -Assume a first order reaction, the rate of the reaction 2A $\rarr$ B is dependent on ______.

Correct Answer
verified

Correct Answer
verified

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the V_max?

Correct Answer
verified

The following questions refer to the overall transformation shown in the following reaction: -For the reaction, the steady state assumption

Correct Answer
verified

When [S] = K_M, $\upsilon$ ₀= (_____) × (V_max) .

Correct Answer
verified

The breakdown of dopamine is catalyzed by the enzyme monoamine oxidase (MAO) .What is the final concentration of product if the starting dopamine concentration is 0.050 M and the reaction runs for 5 seconds.(Assume the rate constant for the reaction is 0.249 s⁻¹.)

Correct Answer
verified

Matching -A lead compound for a new drug should bind to its target protein with a very ______.

Correct Answer
verified

A Lineweaver-Burk plot is also referred to as I.a sigmoidal plot. II.a linear plot. III.a Michaelis-Menten plot. IV.a double reciprocal plot.

Correct Answer
verified

____ clinical trials are focused on evaluating the efficacy of new drug candidates, and usually use _ test.

Correct Answer
verified

Correct Answer
verified

Learning objective: Reaction Kinetics The following questions refer to the diagram (with boxes where it has been left incomplete) : -Which of the following is correct in regards to the diagram above?

Correct Answer
verified

The following questions refer to the overall transformation shown in the following reaction: -Which of the following is (are) true?

Correct Answer
verified

K_M is

Correct Answer
verified

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the K_M?

Correct Answer
verified

Determine the K_M and V_max from the following graph.(Note: On the x-axis the minor tick mark spacing is 0.005; on the y-axis the minor tick mark spacing is 0.002)

Correct Answer
verified

Pseudo-first-order reaction kinetics would be observed for the reaction A + B $\rarr$ C

Correct Answer
verified

Enzyme activity in cells is controlled by which of the following? I.covalent modifications II.modulation of expression levels III.feedback inhibition IV.allosteric effectors

Correct Answer
verified

An enzyme is near maximum efficiency when

Correct Answer
verified

Matching -Different enzymes that catalyze the same reaction, although may be found in different tissues, are known as ______.

Correct Answer
verified

Exam 12: Enzyme Kinetics, Inhibition and Control

Learning objective: Reaction Kinetics The following questions refer to the diagram (with boxes where it has been left incomplete) : -This diagram refers to a (an)

Correct AnswerverifiedShow Answer

Matching -Assume a first order reaction, the rate of the reaction 2A →\rarr→ B is dependent on ______.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the Vmax?

Correct AnswerverifiedShow Answer

The following questions refer to the overall transformation shown in the following reaction: -For the reaction, the steady state assumption

Correct AnswerverifiedShow Answer

When [S] = KM, υ\upsilonυ 0 = (_____) × (Vmax) .

Correct AnswerverifiedShow Answer

The breakdown of dopamine is catalyzed by the enzyme monoamine oxidase (MAO) .What is the final concentration of product if the starting dopamine concentration is 0.050 M and the reaction runs for 5 seconds.(Assume the rate constant for the reaction is 0.249 s−1.)

Correct AnswerverifiedShow Answer

Matching -A lead compound for a new drug should bind to its target protein with a very ______.

Correct AnswerverifiedShow Answer

A Lineweaver-Burk plot is also referred to as I.a sigmoidal plot. II.a linear plot. III.a Michaelis-Menten plot. IV.a double reciprocal plot.

Correct AnswerverifiedShow Answer

________ clinical trials are focused on evaluating the efficacy of new drug candidates, and usually use _____ test.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Learning objective: Reaction Kinetics The following questions refer to the diagram (with boxes where it has been left incomplete) : -Which of the following is correct in regards to the diagram above?

Correct AnswerverifiedShow Answer

The following questions refer to the overall transformation shown in the following reaction: -Which of the following is (are) true?

Correct AnswerverifiedShow Answer

KM is

Correct AnswerverifiedShow Answer

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the KM?

Correct AnswerverifiedShow Answer

Determine the KM and Vmax from the following graph.(Note: On the x-axis the minor tick mark spacing is 0.005; on the y-axis the minor tick mark spacing is 0.002)

Correct AnswerverifiedShow Answer

Pseudo-first-order reaction kinetics would be observed for the reaction A + B →\rarr→ C

Correct AnswerverifiedShow Answer

Enzyme activity in cells is controlled by which of the following? I.covalent modifications II.modulation of expression levels III.feedback inhibition IV.allosteric effectors

Correct AnswerverifiedShow Answer

An enzyme is near maximum efficiency when

Correct AnswerverifiedShow Answer

Matching -Different enzymes that catalyze the same reaction, although may be found in different tissues, are known as ______.

Correct AnswerverifiedShow Answer

Correct Answer
verified

Matching -Assume a first order reaction, the rate of the reaction 2A $\rarr$ B is dependent on ______.

Correct Answer
verified

Correct Answer
verified

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the V_max?

Correct Answer
verified

Correct Answer
verified

When [S] = K_M, $\upsilon$ ₀= (_____) × (V_max) .

Correct Answer
verified

The breakdown of dopamine is catalyzed by the enzyme monoamine oxidase (MAO) .What is the final concentration of product if the starting dopamine concentration is 0.050 M and the reaction runs for 5 seconds.(Assume the rate constant for the reaction is 0.249 s⁻¹.)

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

____ clinical trials are focused on evaluating the efficacy of new drug candidates, and usually use _ test.

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

K_M is

Correct Answer
verified

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the K_M?

Correct Answer
verified

Determine the K_M and V_max from the following graph.(Note: On the x-axis the minor tick mark spacing is 0.005; on the y-axis the minor tick mark spacing is 0.002)

Correct Answer
verified

Pseudo-first-order reaction kinetics would be observed for the reaction A + B $\rarr$ C

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified